Human specific CFTR antibodies detect in vivo gene transfer to ovine airways
نویسندگان
چکیده
1. Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, UK. 2. The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin EH25 9RG, UK. 3. Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK. 4. UK Cystic Fibrosis Gene Therapy Consortium. 5. Centro de Genética Humana, Instituto Nacional Saúde Dr Ricardo Jorge, Lisboa. 6. Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisboa
منابع مشابه
Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
A panel of 11 human cystic fibrosis transmembrane conductance regulator (hCFTR) antibodies were tested in ovine nasal, tracheal, and bronchial epithelial brushings. Two of these, G449 (polyclonal) and MATG1104 (monoclonal), recognized hCFTR but did not cross react with endogenous sheep CFTR. This specificity allows immunologic detection of hCFTR expressed in gene transfer studies in sheep again...
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